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Product Info Summary
| SKU: | M00154-2 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD19 Rabbit Monoclonal Antibody, Clone#RM332
SKU/Catalog Number
M00154-2
Size
100uL
Form
Liquid
Description
Boster Bio Anti-CD19 Rabbit Monoclonal Antibody, Clone#RM332 (Catalog # M00154-2). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD19 Rabbit Monoclonal Antibody, Clone#RM332 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00154-2)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM332
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the C-terminus of human CD19.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to the cytoplasmic domain of human CD19.
Reactive Species
M00154-2 is reactive to CD19 in Human
Applications
M00154-2 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
61.128kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:500-1:1000 dilution
WB: 1:1000-1:2000 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of Raji cell lysate using anti-CD19 rabbit monoclonal antibody (Clone RM332) at a 1:1000 dilution.
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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human Tonsil tissue section using anti-CD19 rabbit monoclonal antibody (Clone RM332) at a 1:1000 dilution.
Protein Target Info & Infographic
Gene/Protein Information For CD19 (Source: Uniprot.org, NCBI)
Gene Name
CD19
Full Name
B-lymphocyte antigen CD19
Weight
61.128kDa
Alternative Names
B4; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; CD19 antigen; CD19 molecule; CD19; CVID3; Differentiation antigen CD19; Leu-12; MGC12802; T-cell surface antigen Leu-12 CD19 B4, CVID3 CD19 molecule B-lymphocyte antigen CD19|B-lymphocyte surface antigen B4|T-cell surface antigen Leu-12|differentiation antigen CD19
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD19, check out the CD19 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD19: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD19 Rabbit Monoclonal Antibody, Clone#RM332 (M00154-2)
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No publications found for M00154-2
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-CD19 Rabbit Monoclonal Antibody, Clone#RM332
Question
I am looking for using your anti-CD19 Rabbit Monoclonal antibody, Clone#RM332 for positive regulation of release of sequestered calcium ion into cytosol studies. Has this antibody been tested with western blotting on raji cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
We appreciate your inquiry. This M00154-2 anti-CD19 Rabbit Monoclonal antibody, Clone#RM332 is tested on raji cell lysate, human tonsil tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-02-28
Question
I was wanting to use your anti-CD19 Rabbit Monoclonal antibody, Clone#RM332 for IHC for human b-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human b-cell identification?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
You can see on the product datasheet, M00154-2 anti-CD19 Rabbit Monoclonal antibody, Clone#RM332 has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human b-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-23
Question
We are currently using anti-CD19 Rabbit Monoclonal antibody, Clone#RM332 M00154-2 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
The anti-CD19 Rabbit Monoclonal antibody, Clone#RM332 (M00154-2) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-22
Question
I am looking for to test anti-CD19 Rabbit Monoclonal antibody, Clone#RM332 M00154-2 on human b-cell for research purposes, then I may be interested in using anti-CD19 Rabbit Monoclonal antibody, Clone#RM332 M00154-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
The products we sell, including anti-CD19 Rabbit Monoclonal antibody, Clone#RM332 M00154-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-31
Question
Is a blocking peptide available for product anti-CD19 Rabbit Monoclonal antibody, Clone#RM332 (M00154-2)?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
We do provide the blocking peptide for product anti-CD19 Rabbit Monoclonal antibody, Clone#RM332 (M00154-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-16
Question
Our lab used your anti-CD19 Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2018-12-05
Answer
I viewed the website and datasheets of our anti-CD19 Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2018-12-05
Question
Do you have a BSA free version of anti-CD19 Rabbit Monoclonal antibody, Clone#RM332 M00154-2 available?
Verified Customer
Verified customer
Asked: 2018-08-31
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD19 Rabbit Monoclonal antibody, Clone#RM332 M00154-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-08-31
Question
I see that the anti-CD19 Rabbit Monoclonal antibody, Clone#RM332 M00154-2 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-07-24
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-07-24
Question
We have observed staining in human tonsil. Are there any suggestions? Is anti-CD19 Rabbit Monoclonal antibody, Clone#RM332 supposed to stain tonsil positively?
Verified Customer
Verified customer
Asked: 2018-06-15
Answer
From literature tonsil does express CD19. From Uniprot.org, CD19 is expressed in spleen, tonsil, blood, b-cell, embryonic kidney, among other tissues. Regarding which tissues have CD19 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 15489334
Blood, Pubmed ID: 1370948
Embryonic kidney, Pubmed ID: 17525332
Spleen, Pubmed ID: 14702039
Tonsil, Pubmed ID: 2472450, 1375324
Boster Scientific Support
Answered: 2018-06-15
Question
Does anti-CD19 Rabbit Monoclonal antibody, Clone#RM332 M00154-2 work for IHC with b-cell?
K. Carter
Verified customer
Asked: 2017-12-04
Answer
According to the expression profile of b-cell, CD19 is highly expressed in b-cell. So, it is likely that anti-CD19 Rabbit Monoclonal antibody, Clone#RM332 M00154-2 will work for IHC with b-cell.
Boster Scientific Support
Answered: 2017-12-04
Question
See attached the WB image, lot number and protocol we used for b-cell using anti-CD19 Rabbit Monoclonal antibody, Clone#RM332 M00154-2. Please let me know if you require anything else.
D. Krishna
Verified customer
Asked: 2017-05-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-31
Question
Is this M00154-2 anti-CD19 Rabbit Monoclonal antibody, Clone#RM332 reactive to the isotypes of CD19?
R. Carter
Verified customer
Asked: 2017-04-26
Answer
The immunogen of M00154-2 anti-CD19 Rabbit Monoclonal antibody, Clone#RM332 is A peptide corresponding to the C-terminus of human CD19. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-04-26
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for b-cell using anti-CD19 Rabbit Monoclonal antibody, Clone#RM332 M00154-2. Let me know if you need anything else.
D. Lewis
Verified customer
Asked: 2015-12-09
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-12-09
Question
Can you help my question with product M00154-2, anti-CD19 Rabbit Monoclonal antibody, Clone#RM332. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Roberts
Verified customer
Asked: 2014-09-03
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00154-2 anti-CD19 Rabbit Monoclonal antibody, Clone#RM332, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-09-03
Question
Does M00154-2 anti-CD19 Rabbit Monoclonal antibody, Clone#RM332 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
T. Krishna
Verified customer
Asked: 2014-06-02
Answer
As indicated on the product datasheet, M00154-2 anti-CD19 Rabbit Monoclonal antibody, Clone#RM332 as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-06-02
Question
My boss were satisfied with the WB result of your anti-CD19 Rabbit Monoclonal antibody, Clone
V. Brown
Verified customer
Asked: 2013-01-10
Answer
From literature, blood does express CD19. Generally CD19 expresses in cell membrane. Regarding which tissues have CD19 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 15489334
Blood, Pubmed ID: 1370948
Embryonic kidney, Pubmed ID: 17525332
Spleen, Pubmed ID: 14702039
Tonsil, Pubmed ID: 2472450, 1375324
Boster Scientific Support
Answered: 2013-01-10
